Biology Services

The structural biology team provides the following functional services that help to accelerate small-molecule drug discovery and have a track record serving more than 20 pharmaceutical/biotech companies worldwide.

Recombinant Protein Expression and Purification Services

Jubilant Biosys offers high-quality proteins for enzymatic assays, high throughput screening, biophysical and structural studies. The team has worked on ~80 protein targets and delivered more than 300 proteins. Recombinant protein services helped customers’ need for high throughput screening activity.

• Constructs design
• Screening in various expression systems (E. coli, baculo-insect and mammalian cells)
• Expression optimization and scale-up
• Co-expression with chaperons or regulatory domain
• Refolding from inclusion bodies
• Various chromatography steps with highly efficient AKTA purification systems
• Affinity chromatography
• Ion exchange chromatography
• Size exclusion chromatography
• Cell culture in flasks and wave bags (5L)
• Purity >95% (on SDS-PAGE)

Biophysical Analysis Services

• Thermal shift assay
• Protein’s melting temperature measurement (Tm)
• Identification of suitable protein storage buffer
• Assess compound binding with protein
• Mass spectrometry (currently being outsourced to IISc, Bangalore)
  • Intact mass measurement
  • Protein identity
• Surface plasmon resonance
  • Pioneer FE system in place
  • Kon, koff & KD in a single injection
  • Evaluation of irreversible inhibition by kinact/Kl or kon Cc
  • Screening low Mw fragments (~125Da)

X-ray Crystal Structural Studies Services

The Jubilant Biosys team has demonstrated its strength in protein engineering to suit the needs of crystallization by designing multiple constructs for a given gene-to-structure project and worked on ~40 different protein targets and delivered more than 250 co-crystal structures (better than 2.5Å resolution). The structural work has been showcased through publications in peer-reviewed international journals (where the Biosys team is co-authored with clients).

• Protein crystallization (apo or in presence of ligand) crystallization screening using commercial kits
• Crystal hit optimization (sparse matrix in-house grid, additive and detergent screening)
• Diffraction data collection using in-house x-rays
• Synchrotron beam access (at Australia)
• Structure determination
• Evaluation of electron density maps for ligand binding
• Structural analysis

Stable Cell Lines Generation Services

Jubilant Biosys has the experience in handling target genes that encompass kinases, carboxylases, ion-channels, GPCRs, lipases, etc. in generating stables in different cell backgrounds by using plasmid-mediated or lentiviral mediated transfection.