Structural Biology

The highly-experienced structural biology team at Jubilant Biosys supports structure-based drug discovery from gene to protein co-crystal structures with small-molecule compounds at high resolution using x-ray crystallography.

Jubilant Biosys provides protein expression and protein purification services in three different expression systems. Our service comes with a full package – from construct design, expression, custom protein production, protein characterization to crystal hit screening and optimization, x-ray diffraction data collection and structure solving. We also provide recombinant proteins and stable cells expressing target proteins for functional assays.

The structural biology team provides the following functional services that help to accelerate small-molecule drug discovery and have a track record serving more than 20 pharmaceutical/biotech companies worldwide.

Recombinant Protein Expression and Purification Services

Jubilant Biosys offers high-quality proteins for biophysical and biochemical assays, high throughput screening, and structural studies. The team has worked on ~80 protein targets and delivered more than 300 proteins. Recombinant protein services helped customers’ need for high throughput screening activity.

  • Constructs design.
  • Screening in various expression systems (E. coli, baculo-insect and mammalian cells).
  • Expression optimization and scale-up.
  • Multi protein co-expression.
    • Obligate protein complexes.
    • With chaperones for enhanced solubility.
    • With regulatory proteins for post-translational modification.
  • Various chromatography steps with highly efficient FPLC purification systems.
    • Affinity chromatography.
    • Ion exchange chromatography.
    • Size exclusion chromatography.
  • Refolding from inclusion bodies.
  • Protein QC.
    • Purity >95% (on SDS-PAGE).
    • Western blots.
    • Intact mass.
  • Large scale Cell culture in shake flasks.

Biophysical Analysis Services

  • Dye-based Thermal shift assay.
    • Protein’s melting temperature measurement (Tm).Identification of suitable protein storage buffer.Assessment of ligand binding.
  • Mass spectrometry (DMPK) .
    • Intact mass measurement.
    • Protein identity and post-translational modifications.
  • Surface plasmon resonance.
    • Sartorius FE system in place. kon, koff& KD in a single injection. Evaluation of irreversible inhibition by kinact/Kl or kon. Fragment Screening of low MW fragments (~125Da).

X-ray Crystal Structural Studies Services

The Jubilant Biosys team has demonstrated its strength in protein engineering to suit the needs of crystallization by designing multiple constructs for a given gene-to-structure project and worked on ~40 different protein targets and delivered more than 250 co-crystal structures (better than 2.5Ã… resolution). The structural work has been showcased through publications in peer-reviewed international journals (where the Biosys team is co-authored with clients).

  • Protein crystallization (apo or in presence of ligand) crystallization screening using commercial kits.
    • Crystal hit optimization (sparse matrix in-house grid, additive and detergent screening).
  • Diffraction screening and data collection using in-house x-rays.
  • Synchrotron beam access (at Australia).
    • Structure determination.
    • Structural analysis.
    • Evaluation of electron density maps for ligand binding.

Stable Cell Lines Generation Services

Jubilant Biosys has the experience in handling target genes that encompass kinases, carboxylases, ion-channels, GPCRs, lipases, etc. in generating stables in different cell backgrounds by using plasmid-mediated transfection.