In Vitro Biology

The In Vitro Biology Group at Jubilant has state-of-the-art infrastructure and the expertise to perform a variety of biochemical and cellular assays against most target classes, including enzymes, GPCR’s, ion channels (e-phys), NHR’s and transporters.

The department is equipped with state-of-the-art facilities to enable these assays, including sophisticated liquid-handling, compound-handling and assay-detection systems. With a high ratio of PhD scientists, the group also has the expertise to take on target validation and mechanism studies.

  • Biochemical & cell based assay development & validation
  • State-of-the-art instrumentation for screening
  • Fully automated compound management system
  • Statistical support with IT enabled screening tools
  • Advanced algorithms for data processing and quality evaluation

Biology Assay Development & Validation

Biochemical and Cell Based Assays

Biosys  
  • ELISA
  • Radioactive Binding Assays
  • FRET
  • HTRF
  • Kinetic Assays
  • Filter Binding Assays
  • Scintillation Proximity Assays
  • Calcium dye based FLIPR and FDSS assays
  • hERG Assays
  • Cell based ELISA
  • Whole Blood Assays
  • Cell Viability (LDH, MTT, Alamar Blue)
  • Flow Cytometry Assays
  • Western Blotting and In Cell ELISA

Target or Pathway Specific Assay

  • DiscoverX Platform, including Beta-arrest in Assays (PathHunter and PathFinder Assays)

Phenotypic Screens

 

Assay Technologies

Biosys  
  • Radiometric- SPA, Filter Binding, Flash Plate
  • Fluorescent- FP, FRET, HTRF, TRF, DELFIA, Lance
  • Luminescent Alpha Screen
  • Luminescent aequorin assays
  • HT Planar Patch Clamp Electrophysiology
  • HT Atomic Absorption Spectroscopy
  • Meso Scale Discovery Platform